Generation and analysis of expressed sequence tags from the medicinal plant Salvia miltiorrhiza

作     者：SPENCER David F 

作者机构：Department of Biochemistry and Molecular BiologyDalhousie University 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2010年第53卷第2期

页      面：273-285页

核心收录：

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 10[医学] 

基　　金：Supported by the 10th and 11th “five-year-technique-project” of Ministry of Science and Technology of China (Grant Nos. 2004BA701A35 and 2006BAI06A12-04) 

主　　题：expressed sequence tags Salvia miltiorrhiza Bge. gene identification microsatellite 

摘      要：Salvia miltiorrhiza *** a well-known traditional Chinese *** roots have been formulated and used clinically for the treatment of various ***,little genetic information has so far been available and this fact has become a major obstacle for molecular *** address this lack of genetic information,an Expressed Sequence Tag (EST) library from whole plantlets of *** was *** the 12959 cDNA clones that were randomly selected and subjected to single-pass sequencing from their 5′ ends,10288 ESTs (with sizes≥100 bp) were selected and assembled into 1288 contigs,leaving 2937 singletons,for a total of 4225 *** were analyzed using BLASTX (against protein databases),RPS-BLAST (against a conserved domain database) as well as the web-based KEGG Automatic Annotation Server for metabolic enzyme *** on the metabolic enzyme assignment,expression patterns of 14 secondary metabolic enzyme genes in different organs and under different treatments were verified using real-time PCR ***,a total of 122 microsatellites were identified from the ESTs,with 89 having sufficient flanking sequences for primer *** set of ESTs represents a significant proportion of the *** transcriptome,and gives preliminary insights into the gene complement of *** will prove useful for uncovering secondary metabolic pathways,analyzing cDNA-array based gene expression,genetic manipulation to improve yield of desirable secondary products,and molecular marker identification.