Development of a sandwich ELISA for the detection of bovine herpesvirus type 1

作     者：Shanaz Bashir Rashmi Singh Barkha Sharma Sharad K Yadav 

作者机构：Department of Epidemiology and Vety.Preventive medicineCollege of Veterinary SciencesVeterinary UniversityMathura(U.P.)India 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第5期

页      面：363-366页

核心收录：

学科分类：090603[农学-临床兽医学] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 

基　　金：DUVASU Jeju National University 

主　　题：Bovine herpesvirus type 1 Antisera Enzyme-linked immunosorbent assay (ELISA) 

摘      要：Objective:To develop a standard enzyme-linked immunosorbent assay（ELJSA） for the detection of bovine herpesvirus type 1（BHV-1）.Methods:The assay was based on hyperimmune rabbit and guinea pig antisera raised against purified *** glycol precipitation and sucrose density gradient methods were adopted for viral concentration and *** were raised using Freund’s adjuvant followed by extraction of IgG of high ***: Optimum antisera dilutions as determined by titrations were chosen as 14 000,whereas the conjugate was used at 1:2 000 *** 95 clinical specimens,the ELISA test showed a sensitivity and specificity of 91.90%and 93.10%,respectively when compared to *** cutoff value was fixed at 0.15490) and a P/N ratio of1.30 indicated a significant positive reaction. Conclusions:The results have demonstrated that this ELISA could efficiently detect BHV-1 and can be used as an important diagnostic tool.