Isolation of a 1 195 bp 5′-Flanking Region of Rice Cytosolic Fructose-1,6-bisphosphatase and Analysis of Its Expression in Transgenic Rice

作     者：司丽珍 曹守云 储成才 SI Li-Zhen;CAO Shou-Yun;CHU Cheng-Cai

作者机构：中国科学院遗传与发育生物学研究所北京100101 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2003年第45卷第3期

页      面：359-364页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 

基　　金：国家自然科学基金 ( 3 0 170 492 )~~ 

主　　题：rice (Oryza sativa) promoter cytosolic fructose-1, 6-bisphosphatase gene mesophyll-specific expression 

摘      要：A genomic DNA fragment containing the 5 -upstream sequence and part of the open reading frame corresponding to the cytosolic fructose-1,6-bisphosphatase (cyFBPase) cDNA was isolated by Genome Walking. The 1 195 lip 5 -flanking region which started from the translation initiation ATG codon was fused to reporter gene encoding beta-glucuronidase (GUS) and stably transferred to rice via particle bombardment. Strong GUS activity was detected in leaves and leaf sheaths of transgenic rice, but not in culms and roots. Histochemical localization revealed that GUS expression was exclusively restricted to mesophyll cells in transgenic rice. Our results indicate that the 1 195 bp fragment contains all the cis-elements required for directing mesophyll-specific expression pattern in rice.