Phyllanthus acidus(L.) Skeels and Rhinacanthus nasutus(L.) Kurz leaf extracts suppress melanogenesis in normal human epidermal melanocytes and reconstitutive skin culture

作     者：Moragot Chatatikun Takeshi Yamauchi Kenshi Yamasaki Anchalee Chiabchalard Setsuya Aiba 

作者机构：Department of Medical Technology School of Allied Health Sciences Walailak University Department of Dermatology Tohoku University Graduate School of Medicine Deparment of Clinical Chemistry Faculty of Allied Health Sciences Chulalongkorn University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2019年第12卷第3期

页      面：98-105页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

基　　金：financially supported by by research grant from Kao Melanin Workshop(KY) by Grant-in-Aid for Challenging Exploratory Research 16K15542(KY) a Grant-in-aid for Scientific Research C 24591622(KY) from the Ministry of Education,Culture,Sports,Science and Technology,Japan by Novartis Pharma Research Grants(KY) by grants from the Department of Dermatology,Tohoku University Graduate School of Medicine,Japan 

主　　题：Phyllanthus acidus(L.) Skeels Rhinacanthus nasutus(L.) Kurz Melanin Tyrosinase MITF 

摘      要：Objective: To determine the effect of extracts from Phyllanthus acidus(P. acidus)(L.) Skeels and Rhinacanthus nasutus(R. nasutus)(L.) Kurz leaves on melanogenesis and the underlying mechanism in normal human epidermal melanocytes(NHEM) and a reconstitutive skin model. Methods: NHEM and a reconstitutive skin model were stimulated with ethanol extracts of P. acidus(L.) Skeels and R. nasutus(L.) Kurz leaves. mRNA expression of microphthalmiaassociated transcription factor(MITF), tyrosinase(TYR), tyrosinase-related protein 1(TYRP1) and dopachrome tautomerase(DCT) were examined by real-time PCR. The melanin content in NHEM was also measured. Moreover, protein levels of tyrosinase were determined using western blot ***: In NHEM and the reconstitutive skin model, ethanol extracts from P. acidus(at 12.5 and 25.0 μg/mL) and R. nasutus(at 6.25 and 12.50 μg/mL) significantly diminished mRNA expression of MITF, TYR, TYRP1 and DCT in a concentration-dependent manner. P. acidus and R. nasutus extracts also reduced the amount of melanin in α-MSH-stimulated NHEM. Moreover, P. acidus and R. nasutus extracts markedly suppressed tyrosinase at the translational level in the reconstitutive skin model. Conclusions: P. acidus and R. nasutus extracts significantly reduced melanogenesis in NHEM and the reconstitutive skin model, suggesting that P. acidus and R. nasutus extracts can inhibit melanin synthesis through downregulation of MITF, TYR, TYRP1 and DCT. Therefore, the ethanol extracts of P. acidus and R. nasutus contain compounds that have the potential for development as a skin lightening agent for the treatment of hyperpigmentation disorder or melasma.