A new interaction between Abi-1 and βPIX involved in PDGF-activated actin cytoskeleton reorganisation

作     者：Fanny Campa NikolausMachuy AlexanderKlein ThomasRudel 

作者机构：Max 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2006年第16卷第9期

页      面：759-770页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：βPIX Abi-1 PDGF membrane dorsal ruffling cytoskeleton 

摘      要：Members of the Rho family of GTPases are key regulators of the actin *** particular,activated Rac 1stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor (PDGF).Abl-interactor (Abi)-1 and βPIX,a guanine nucleotide exchange factor for Rac 1,localise at these Rac1-induced actin structures and playimportant roles in the induction of membrane dorsal ruffling in response to PDGF in ***,we demonstratea novel interaction between Abi-1 and βPIX using the yeast two-hybrid system,in vitro pull-down assays,and in vivoco-immunoprecipitation *** vitro,the C-terminal fragment of ]3PIX interacted with Abi-1,while in vivo theN-terminal fragment of βPIX interacted with *** biological function of this interaction was investigated in mousefibroblasts in response to PDGF ***-1 and βPIX co-localised in the cytoplasm and to membrane dorsalruffles after PDGF *** show that the co-expression of Abi-1 and truncated forms of βPIX in mouse fibroblastsblocked PDGF-induced membrane dorsal ***,these results show that the interaction between Abi-1 andβPIX is involved in the formation of growth factor-induced membrane dorsal ruffles.