Combined effects of water temperature and copper ion concentration on catalase activity in Crassostrea ariakensis

作     者：王辉 杨洪帅 刘加慧 李艳红 刘志刚 

作者机构：School of Life Sciences of Huaiyin Normal University Fisheries College of Guangdong Ocean University 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2015年第33卷第4期

页      面：905-912页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the Guangdong Province Science & Technology Project(No.2010B020201014) the Guangdong Province Education Department(No.GCZX-A0909) the Guangdong Province Ocean and Fisheries Science & Technology Extension Project(No.20120980) the Guangdong Province Industry-University-Science Partnership Project(No.20110908) the Sci & Tech Plan of Huaiyin Normal University(No.00wh0031) 

主　　题：Crassostrea ariakensis catalase temperature copper ion concentration combined effects 

摘      要：A central composite experimental design and response surface method were used to investigate the combined effects of water temperature(18–34℃) and copper ion concentration(0.1–1.5 mg/L) on the catalase(CAT) activity in the digestive gland of C rassostrea ariakensis. The results showed that the linear effects of temperature were significant(P 0.05), and the quadratic effects of copper ion concentration were significant(P 0.05), and the effect of temperature was greater than that of copper ion concentration. A model equation of CAT enzyme activity in the digestive gland of C. ariakensis toward the two factors of interest was established, with R 2, Adj. R 2 and Pred. R 2 values as high as 0.943 7, 0.887 3 and 0.838 5, respectively. These findings suggested that the goodness of fit to experimental data and predictive capability of the model were satisfactory, and could be practically applied for prediction under the conditions of the study. Overall, the results suggest that the simultaneous variation of temperature and copper ion concentration alters the activity of the antioxidant enzyme CAT by modulating active oxygen species metabolism, which may be utilized as a biomarker to detect the effects of copper pollution.