In vivo superresolution photoacoustic computed tomography by localization of single dyed droplets

作     者：Pengfei Zhang Lei Li Li Lin Junhui Shi Lihong V.Wang 

作者机构：Optical Imaging LaboratoryDepartment of Biomedical EngineeringWashington University in St.LouisSt.LouisMissouri 63130USA Caltech Optical Imaging LaboratoryAndrew and Peggy Cherng Department of Medical EngineeringDepartment of Electrical EngineeringCalifornia Institute of Technology1200 E.California Blvd.MC 138-78PasadenaCA 91125USA Present address:School of Precision Instruments and Optoelectronics EngineeringTianjin UniversityTianjin 300072China 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2019年第8卷第1期

页      面：884-892页

核心收录：

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

基　　金：sponsored by the NIH grants DP1 EB016986(NIH Director’s Pioneer Award) R01 CA186567(NIH Director’s Transformative Research Award) U01 NS090579(BRAIN Initiative) U01 NS099717(BRAIN Initiative) 

主　　题：technique. resolution dye 

摘      要：Photoacoustic(PA)computed tomography(PACT)is a noninvasive hybrid imaging technique that combines optical excitation and acoustic detection to realize high contrast,high resolution,and deep penetration in biological ***,the spatial resolution of PACT is limited by acoustic ***,we report in vivo superresolution PACT,which breaks the acoustic diffraction limit by localizing the centers of single dyed droplets that are flowing in blood *** droplets were prepared by dissolving hydrophobic absorbing dye in oil,followed by mixing with *** dyed droplets generate much higher-amplitude PA signals than blood and can flow smoothly in vessels;thus,they are excellent tracers for localization-based superresolution *** in vivo resolution enhancement was demonstrated by continuously imaging the cortical layer of a mouse brain during droplet *** droplets that were flowing in the vessels were localized,and their center positions were used to construct a superresolution image that exhibits sharper features and more finely resolved vascular *** improvement in spatial resolution by a factor of 6 has been realized in vivo by the droplet localization technique.