Peptide nucleic acid (PNA) binding-mediated gene regulation

作     者：Gan WANG, Xiaoxin SXU Institute of Environmental Health Sciences, Wayne State University, 2727 Second Avenue, Detroit, MI 48201, USA Gan WANG, Xiaoxin SXU Institute of Environmental Health Sciences, Wayne State University, 2727 Second Avenue, Detroit, MI 48201, USA

作者机构：1Institute of Environmental Health Sciences Wayne State University 2727 Second Avenue Detroit MI 48201 USA 1Institute of Environmental Health Sciences Wayne State University 2727 Second Avenue Detroit MI 48201 USA 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2004年第14卷第2期

页      面：111-116页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

主　　题：peptide nucleic acids (PNAs) PNA binding single-stranded D-loop transcription initiation. 

摘      要：Peptide nucleic acids （PNAs） are synthetic oligonucleotides with chemically modified backbones. PNAs can bind to both DNA and RNA targets in a sequence-specific manner to form PNA/DNA and PNA/RNA duplex structures. When bound to double-stranded DNA （dsDNA） targets, the PNA molecule replaces one DNA strand in the duplex by strand invasion to form a PNA/DNA/PNA [or （PNA）2/DNA] triplex structure and the displaced DNA strand exists as a singlestranded D-loop. PNA has been used in many studies as research tools for gene regulation and gene targeting. The Dloops generated from the PNA binding have also been demonstrated for its potential in initiating transcription and inducing gene expression. PNA provides a powerful tool to study the mechanism of transcription and an innovative strategy to regulate target gene expression. An understanding of the PNA-mediated gene regulation will have important clinical implications in treatment of many human diseases including genetic, cancerous, and age-related diseases.