A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root
作者机构：National Key Laboratory of Plant Molecular Genetics(NKLPMG)CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and Ecology(SIPPE)Shanghai 200032P.R.China University of Chinese Academy of SciencesShanghai 200032P.R.China ShanghaiTech UniversityShanghai 200031P.R.China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2019年第12卷第5期
页 面：648-660页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学]
基 金：the State Key Basic Research Program of China(2013CB127000) National Natural Science Foundation of China(31430013 31222029 912173023,31525004) Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27030101) Young Elite Scientists Sponsorship Program by CAST(2016QNRC001) National Postdoctoral Program for Innovative Talents(BX201600178) the Sanofi-SI BS 2017 Post-doctoral Fellowship
主 题：Arabidopsis root scRNA-seq cell atlas differentiation trajectory meristem
摘 要：Cells of eukaryotic multicellular organisms have inherent *** advances in single-cell gene expression studies enable us to explore transcriptional regulation in dynamic development processes and highly heterogeneous cell *** this study,using a high-throughput single-cell RNA-sequencing assay,we found that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes.A total of 24 putative cell clusters and the cluster-specific marker genes were *** spatial distribution and temporal ordering of the individual cells at different developmental stages illustrate their hierarchical structures and enable the reconstruction of continuous differentiation trajectory of root ***,we found that each root cell cluster exhibits distinct patterns of ion assimilation and hormonal ***,our study reveals a high degree of heterogeneity of root cells and identifies the expression signatures of intermediate states during root cell differentiation at single-cell *** also established a web server(http://***/rootatlas/)to facilitate the use of the datasets generated in this study.
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