Poxue Huayu and Tianjing Busui Decoction for cerebral hemorrhage Upregulation of neurotrophic factor expression

作     者：Jixiang Ren Xiangyu Zhou Jian Wang Jianjun Zhao Pengguo Zhang 

作者机构：Department of Encephalopathythe Affiliated Hospital to Changchun University of Chinese Medicine Grade 2010 Clinical Medicine MajorSchool of Clinical MedicineYanbian University Department of ImagingSchool of Second Clinical MedicineJilin University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第22期

页      面：2039-2049页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Chinese Medicine Research Center Foundation (Stroke),No.2012B02 Jilin Provincial Natural Science Foundation in China,No.201015211 

主　　题：neural regeneration traditional Chinese medicine cerebral hemorrhage brain-derived neurotrophicfactor tyrosine kinase B vascular endothelial growth factor Poxue Huayu and Tianjing Busui grants-supported paper neuroregeneration 

摘      要：This study established a rat model of cerebral hemorrhage by injecting autologous anticoagulated blood. Rat models were intragastrically administered 5, 10, 20 g/kg Poxue Huayu and Tianjing Busui Decoction, supplemented with Hirudo, raw rhubarb, raw Pollen Typhae, gadfly, Fructrs Trichosanthis, Radix Notoginseng, Rhizoma Acori Talarinowii, and glue of tortoise plastron, once a day, for 14 consecutive days. Results demonstrated that brain water content significantly reduced in rats with cerebral hemorrhage, and intracerebral hematoma volume markedly reduced after treat- ment. Immunohistochemical staining revealed that brain-derived neurotrophic factor, tyrosine kinase B and vascular endothelial growth factor expression noticeably increased around the sur- rounding hematoma. Reverse transcription-PCR revealed that brain-derived neurotrophic factor and tyrosine kinase B mRNA expression significantly increased around the surrounding hematoma. Neurologic impairment obviously reduced. These results indicated that Poxue Huayu and Tianjing Busui Decoction exert therapeutic effects on cerebral hemorrhage by upregulating the expression of brain-derived neurotrophic factor.