Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides

作     者：LI YanRong, GAN ZhiYong, LI YanFen, Liu Qian, BAO JianChun, DAI ZhiHui & HAN Min Jiangsu Key Laboratory of Biofunctional Materials College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China 

作者机构：Jiangsu Key Laboratory of Biofunctional Materials Nanjing Normal University Nanjing China College of Chemistry and Environmental Science Nanjing Normal University Nanjing China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2010年第53卷第4期

页      面：820-825页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 0703[理学-化学] 10[医学] 

基　　金：support from the National Natural Science Foundation of China (Grant Nos. 20875046  20871070 & 20901041) 

主　　题：acetylcholinesterase organophosphorous insecticides immobilization gold 

摘      要：This paper reports a simple method for immobilization of acetylcholinesterase (AChE) on one-dimensional (1D) gold (Au) nanoparticles for detection of organophosphorous (OP) insecticides. 1D Au nanoparticles were prepared by electrodeposition in the pores of an alumina template which was subsequently removed by 2.0 M NaOH solution. They were characterized by XRD and FESEM. The immobilized AChE retained its biological activity and catalyzed the hydrolysis of acetylthiocholine to form thiocholine, which was subsequently oxidized to produce detectable signals. Based on the inhibition toward the enzymatic activity of AChE by OP insecticides, sensitive detection of methamidophos (an OP insecticide) was performed. Under optimal conditions, the sensors could be used for the determination of methamidophos ranging from 0.004 to 24 μg/mL with the detection limit of 0.001 μg/mL. The developed OP insecticide biosensors exhibited satisfactory stability and reproducibility. This work demonstrated that 1D Au nanoparticles could serve as an ideal carrier for immobilization of AChE to fabricate the corresponding biosensor.