Localization Analysis of Heterophilic Antigen Epitopes of H1N1 Influenza Virus Hemagglutinin

作     者：Chun-Yan Guo Hai-Xiang Zhang Jun-Jun Zhang Li-Jun Sun Hui-Jin Li Dao-Yan Liang Qing Feng Yan Li Yang-Meng Feng Xin Xie Jun Hu 

作者机构：Shaanxi Province Research Center of Cell Immunological Engineering and TechnologyCentral Laboratory of Shaanxi Provincial People's HospitalXi'an 710068China Xian Yang Municipal Center for Disease Control and PreventionXianyang 712000China Shaanxi Key Laboratory of Ischemic Cardiovascular DiseaseInstitute of Basic and Translational MedicineXi'an Medical UniversityXi'an 710021China Key Laboratory of Resource Biology and Biotechnology in Western ChinaMinistry of EducationCollege of Life SciencesNorthwest UniversityXi'an 710069China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2019年第34卷第3期

页      面：306-314页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 

基　　金：supported by The National Key Research and Development Program of China (Grant No. 2016YFD0500700) The Natural Science Basic Research Program of Shaanxi Province (Grant No. 2016JM8065) Shaanxi Provincial People’s Hospital Incubation Fund Program (Grant No. 2015YX-4) 

主　　题：H1N1 influenza virus HA antigen Monoclonal antibody Localization Heterophilic epitope 

摘      要：Previous studies have indicated that two monoclonal antibodies(mAbs;A1-10 and H1-84)of the hemagglutinin(HA)antigen on the H1N1 influenza virus cross-react with human brain *** has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue(Guo et *** Immunobiology 220:941-946,2015).However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been *** the present study,the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN *** epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL *** amino acids and variable sequences of the antibodies were identified using abYsis *** results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the *** of the peptides,P2(LVLWGIHHP191-199),bound both of the mAbs and was located in the head region of *** key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described.A heterophilic epitope on H1N1 influenza virus HA was also *** existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection,which might aid in influenza prevention and control.