The lifestyle transition of Arthrobotrys oligospora is mediated by microRNA-like RNAs

作     者：Xinglai Ji Heng Li Weihua Zhang Jiai Wang Lianming Liang Chenggang Zou Zefen Yu Shuqun Liu Ke-Qin Zhang Xinglai Ji;Heng Li;Weihua Zhang;Jiai Wang;Lianming Liang;Chenggang Zou;Zefen Yu;Shuqun Liu;Ke-Qin Zhang

作者机构：State Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanYunnan UniversityKunming 650091China Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu ProvinceNanjing University of Posts and TelecommunicationsNanjing 210023China Institute of Medical BiologyChinses Academy of Medical Sciences&Peking Union Medical CollegeKunming 650118China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2020年第63卷第4期

页      面：543-551页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 071007[理学-遗传学] 

基　　金：This work was supported by the National Basic Research Program of China(2013CB127500) the National Natural Science Foundation of China(31160021,31270131 and U1502262) sponsored by the Nanjing University of Posts and Telecommunications Scientific Foundation(NUPTSF)(NY218140) a grant(2018KF003)from YNCUB.We thank BGI-Shenzhen who contributed to the small RNA sequencing projects.We thank H.Yin for comments and discussion 

主　　题：lifestyle transition Arthrobotrys oligospora,microRNA-like RNAs nematode-trapping fungi trap induction fungal adaptation 

摘      要：The lifestyle transition of fungi,defined as switching from taking organic material as nutrients to pathogens,is a fundamental phenomenon in ***,the mechanisms of such transition remain largely *** we show microRNA-like RNAs(milRNAs)play a key role in fungal lifestyle transition for the first *** identified milRNAs by small RNA sequencing in Arthrobotrys oligospora,a known nematode-trapping *** them,7 highly expressed milRNAs were confirmed by northern-blot *** out two milRNAs significantly decreased ***’s ability to switch *** further identified that two of these milRNAs were associated with argonaute protein QDE-2 by RNA-immunoprecipitation(RIP)*** of the predicted target genes of milRNAs were found in immunoprecipitation(IP)products of *** of argonaute gene qde-2 also led to serious defects in lifestyle ***,knocking out individual milRNAs or qde-2 lead to diverse responses under different conditions,and qde-2 itself may be targeted by the ***,it indicates the lifestyle transition of fungi is mediated by milRNAs through RNA interference(RNAi)machinery,revealing the wide existence of miRNAs in fungi kingdom and providing new insights into understanding the adaptation of fungi from scavengers to predators and the mechanisms underlying fungal infections.