PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions

作     者：Xiaona Huang Chao Wei Fenjie Li Lumeng Jia Pengguihang Zeng Jiahe Li Jin Tan Tuanfeng Sun Shaoshuai Jiang Jia Wang Xiuxiao Tang Bin Liu Limin Rong Cheng Li Junjun Ding 

作者机构：RNA Biomedical InstituteSun Yat-Sen Memorial HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China Center for Stem Cell Biology and Tissue EngineeringKey Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-Sen UniversityGuangzhou 510080China Department of Cell BiologyZhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Program in Stem Cell and Regenerative MedicineThe Third Affiliated Hospital of Sun Yat-sen UniversityZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouChina Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing 100871China Department of Spine SurgeryThe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Histology and EmbryologySchool of Basic Medical SciencesGuangzhou Medical UniversityGuangzhou 511436China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2019年第10卷第10期

页      面：709-725页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funded by grants from the National Key Research and Development Program 国家自然科学基金 the Guangdong Regenerative Medicine and Health of Guangdong Laboratory Frontier Exploration Project the Guangdong Innovative and Entrepreneurial Research Team Program the Fundamental Research Funds for the Central Universities funded by China Postdoctoral Science Foundation to F.L 

主　　题：PCGF6 Polycomb group super-enhancer 3D chromatin pluripotency 

摘      要：Polycomb group(PcG)ring finger protein 6(PCGF6),though known as a member of the transcription-re-pressing complexes,PcG,also has activation function in regulating pluripotency gene ***,the mechanism underlying the activation function of PCGF6 is poorly ***,we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as *** addition,PCGF6 was recruited to a subset of the super-enhancer(SE)regions upstream of cell cycle-associated genes by OCT4,and increased their *** combining with promoter capture Hi-C data,we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D *** fin dings highlight a novel mechanism of PcG protein in regulating pluripotency,and provide a research basis for the therapeutic application of pluripotent stem cells.