Transcriptome analysis provides insights into the regulation of metabolic processes during postharvest cold storage of loquat(Eriobotrya japonica)fruit

作     者：Wenli Liu Jing Zhang Chen Jiao Xueren Yin Zhangjun Fei Qingbiao Wu Kunsong Chen 

作者机构：School of Mathematical ScienceZhejiang UniversityYuquan Campus310027 HangzhouP.R.China Boyce Thompson InstituteCornell UniversityIthacaNY 14853USA Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative BiologyZhejiang UniversityZijingang Campus310058 HangzhouP.R.China USDA-ARS Robert W.Holley Center for Agriculture and HealthIthacaNY 14853USA 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2019年第6卷第1期

页      面：1142-1152页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 070301[理学-无机化学] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）] 

基　　金：supported by grants from the National Natural Science Foundation of China(31630067,11771393,and 11632015) the Natural Science Foundation of Zhejiang Province,China(LR16C150001 and LZ14A010002) the Project of the Science and Technology Department of Zhejiang Province(2016C04001) the US National Science Foundation(IOS-1539831) 

主　　题：insight analysis treatment 

摘      要：Loquat(Eriobotrya japonica)fruit accumulates lignin during postharvest storage under chilling conditions(0℃),while low-temperature conditioning(LTC;5℃for 6 days followed by transfer to 0℃)or heat treatment(HT;40℃for 4 h followed by transfer to 0℃)can alleviate *** we compared transcriptome profiles of loquat fruit samples under LTC or HT to those stored at 0℃at five time points from day 1 to day 8 after ***-throughput transcriptome sequences were de novo assembled into 53,319 unique transcripts with an N50 length of 1306 bp.A total of 2235 differentially expressed genes were identified in LTC,and 1020 were identified in HT compared to 0℃.Key genes in the lignin biosynthetic pathway,including EjPAL2,EjCAD1,EjCAD3,4CL,COMT,and HCT,were responsive to LTC or HT treatment,but they showed different expression patterns during the treatments,indicating that different structural genes could regulate lignification at different treatment *** network analysis showed that these candidate biosynthetic genes were associated with a number of transcription factors,including those belonging to the AP2,MYB,and NAC *** ontology(GO)enrichment analysis of differentially expressed genes indicated that biological processes such as stress responses,cell wall and lignin metabolism,hormone metabolism,and metal ion transport were significantly affected under LTC or HT treatment when compared to 0℃.Our analyses provide insights into transcriptome responses to postharvest treatments in loquat fruit.