The Blue-Light Receptor CRY1 Interacts with BZR1 and BIN2 to Modulate the Phosphorylation and Nuclear Function of BZR1 in Repressing BR Signaling in Arabidopsis

作     者：Guanhua He Jie Liu Huixue Dong Jiaqiang Sun 

作者机构：National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2019年第12卷第5期

页      面：689-703页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS) the Agricultural Science and Technology Innovation Program of CAAS Youth Talent Plan of CAAS 

主　　题：brassinosteroid BIN2 BZR1 cryptochrome 1 

摘      要：The blue-light receptor cryptochrome 1(CRY1)primarily mediates blue-light inhibition of hypocotyl elongation in ***,the underlying mechanisms remain largely *** report here that CRY1 inhibits hypocotyl elongation by repressing brassinosteroid(BR)signaling.A genetic interaction assay reveals the negative regulatory effect of CRY1 on the function of BZR1,a core transcription factor in the BR signaling *** demonstrated that CRY1 interacts with the DNA-binding domain of BZR1 to interfere with the DNA-binding ability of BZR1,and represses its transcriptional ***,we found that CRY1 promotes the phosphorylation of BZR1 and inhibits the nuclear accumulation of ***,we discovered that CRY1 interacts with the GSK3-like kinase BIN2 and enhances the interaction of BIN2 and BZR1 in a light-dependent *** findings revealed that CRY1 negatively regulates the function of BZR1 through at least two mechanisms:interfering with the DNA-binding ability of BZR1 and promoting the phosphorylation of ***,we uncover a novel CRY1-BIN2-BZR1 regulatory module that mediates crosstalk between blue light and BR signaling to coordinate plant growth in Arabidopsis.