Schizonepeta tenuifolia inhibits collagen stimulated platelet function via suppressing MAPK and Akt signaling

作     者：Bo-Ra Jeon Muhammad Irfan Minki Kim Seung Eun Lee Jeong Hoon Lee Man Hee Rhee 

作者机构：Laboratory of Physiology and Cell SignalingCollege of Veterinary MedicineKyungpook National UniversityDaegu 41566Republic of Korea Department of Herbal Crop ResearchNational Institute of Horticultural and Herbal ScienceEumseong 27709Republic of Korea. 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2019年第33卷第4期

页      面：250-257页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Research Foundation of Korea grant funded by the Korean Government(MSIP,No.2015R1D1-AIA09057204) 

主　　题：S.tenuifolia platelet MAPK Akt natural antithrombotic agent 

摘      要：The prevalence of cardiovascular diseases(CVDs)is increasing at a rapid pace in developed countries,and CVDs are the leading cause of morbidity and *** products and ethnomedicine have been shown to reduce the risk of ***(S.)tenuifolia is a medicinal plant widely used in China,Korea,and Japan and is known to exhibit anti-inflammatory,antioxidant,and immunomodulatory *** hypothesized that given herbal plant exhibit pharmacological activities against CVDs,we specifically explored its effects on platelet *** aggregation was evaluated using standard light transmission *** calcium mobilization was assessed using Fura-2/AM,and granule secretion(ATP release)was measured in a *** binding to integrin a_(Ⅱb)β_3,was assessed using flow *** of mitogen-activated protein kinase(MAPK)signaling molecules and activation of the protein kinase B(Akt)was assessed using Western blot ***,extract potently and significantly inhibited platelet aggregation,calcium mobilization,granule secretion,and fibrinogen binding to integrin a_(Ⅱb)β_***,all extracts significantly inhibited MAPK and Akt *** extract inhibited platelet aggregation and granule secretion,and attenuated collagen mediated GPVI downstream signaling,indicating the potential therapeutic effects of these plant extracts on the cardiovascular system and platelet *** suggest that *** extract may be a potent candidate to treat platelet-related CVDs and to be used as an antiplatelet and antithrombotic agent.