Identification of AOSC-binding proteins in neurons

作     者：刘明 聂琴 辛现良 耿美玉 

作者机构：Department of Molecular PharmacologySchool of Medicine and Pharmacy Ocean University of China Division of Anti-tumor PharmacologyState Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2008年第26卷第4期

页      面：394-399页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0908[农学-水产] 0707[理学-海洋科学] 0815[工学-水利工程] 100204[医学-神经病学] 10[医学] 

基　　金：Supported by National Basic Research Program of China (973 Program) (No.2003CB716400) Natural Science Foundation of China for Distingutshed Young Scholars (No.30725046) Natural Science Foundation of China (No.30472029,No.30772612) Chinese High technology Research and Development Program of China(863 Program) (No.2006AA090501) 

主　　题：AOSC Alzheimer's disease marine oligosaccharide binding protein(s) 

摘      要：Acidic oligosaccharide sugar chain （AOSC）, a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer＇s Disease （AD） drug candidate. The identification of AOSC-binding protein（s） in neurons is very important for understanding its action mechanism. To determine the binding protein（s） of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 （AP-2） and amyloid precursor protein （APP）. These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.