Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology
Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology作者机构：Institute of Animal Husbandry and VeterinaryShanxi Academy of Agricultural Sciences
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2019年第8卷第1期
页 面：86-89,92页
学科分类：09[农学]
基 金：Supported by Special Fund for National Hair Sheep Industrial Technology System(CARS-39-24) Science and Technology Development Program of Shanxi Province(20120311024-1) Science and Technology Innovation Team Project of Shanxi Province(201705D131028-20) Financial Support of Agriculture of Shanxi Province(NYGX2015-03) Talent Project for Science and Technology Development in Outlaying Poor Areas,Frontier Ethnic Minority Areas and Old Revolutionary Base Areas of Shanxi Province,China(2017Sy128)
主 题：Juvenile lambs Age Oocytes In vitro culture system JIVET Maturation rate Cleavage rate
摘 要：[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare s serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium.
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