Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment

作     者：Chao LIU Xian ZHANG Zhi-ming RAO Ming-long SHAO Le-le ZHANG Dan WU Zheng-hong XU Hui LI 

作者机构：Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University Laboratory of Applied Microorganisms and Metabolic Engineering School of Biotechnology Jiangnan University Laboratory of Pharmaceutical Engineering School of Pharmaceutical Science Jiangnan University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2015年第16卷第4期

页      面：286-295页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the National Basic Research Program(973)of China(No.2012CB725202) the National High-Tech R&D Program(863)of China(No.2011AA02A211) the National Natural Science Foundation of China(No.21276110) the Fundamental Research Funds for the Central Universities(Nos.JUSRP51306A and JUSRP11545) the National 111 Project of China’s Higher Education(No.111-2-06) the Program of the Key Laboratory of Industrial Biotechnology,Ministry of Education,China(No.KLIB-KF201406) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China 

主　　题：Mycobacterium neoaurum Atmospheric and room temperature plasma (ARTP) Mutation breeding 4-Androstene-3 17-dione (AD) 1 4-Androstadiene-3 17-dione (ADD) 

摘      要：Steroid medication is used extensively in clinical applications and comprises a large and vital part of the pharmaceutical industry. However, the difficulty of separating 4-androstene-3,17-dione （AD） from 1,4-androstadiene- 3,17-dione （ADD） restricts the application of the microbial transformation of phytosterols in the industry. A novel at- mospheric and room temperature plasma （ARTP） treatment, which employs helium as the working gas, was used to generate Mycobacterium neoaurum mutants producing large amounts of AD. After treatment of cultures with ARTP, four mutants were selected using a novel screening method with a color assay. Among the mutants, M. neoaurum ZADF-4 was considered the best candidate for industrial application. When the fermentation medium contained 15 g/L phytosterols and was cultivated on a rotary shaker at 160 r/min at 30 ~C for 7 d, （6.28＋0.11） g/L of AD and （0.82＋0.05） g/L of ADD were produced by the ZADF-4 mutant, compared with （4.83＋0.13） g/L of AD and （2.34＋0.06） g/L of ADD by the original strain, Iv~. neoaururn ZAD. Compared with ZAD, the molar yield of AD increased from 48.3% to 60.3% in the ZADF-4 mutant. This result indicates that ZADF-4 may have potential for industrial production of AD.