A non-invasive,rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms

作     者：Li Yi Ting Wu Wenyuan Luo Wen Zhou Jun Wu 

作者机构：Department of NeurologyPeking University Shenzhen Hospital Department of RadiologyPeking University Shenzhen Hospital 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第1期

页      面：69-75页

核心收录：

学科分类：1002[医学-临床医学] 100203[医学-老年医学] 10[医学] 

基　　金：supported by two grants from Science Industry Trade and Information Technology Commission of Shenzhen Municipality in China grant No.201002063 JC20110518075 7A 

主　　题：nerve regeneration neurodegeneration late-onset Alzheimer's disease apolipoprotein Egene real-time PCR DNA sequencing risk factor allele neural regeneration 

摘      要：The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer＇s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA se- quencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer＇s disease cases.