Specific cellular immune responses in mice immunized with DNA,adeno-associated virus and adenoviral vaccines of Epstein-Barr virus-LMP2 alone or in combination

作     者：WANG Zhan YANG SongMei ZHOU Ling DU HaiJun MO WuNing ZENG Yi 

作者机构：State Key Laboratory for Infectious Disease Prevention and Control Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Laboratory Department The First Affiliated Hospital Guangxi Medical University Nanning 530021 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2011年第54卷第3期

页      面：263-266页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 090602[农学-预防兽医学] 07[理学] 08[工学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National High Technology Research and Development Program of China(Grant No. 2006AA02A229) 

主　　题：EBV-LMP2 DNA vaccine recombinant adenovirus vaccine recombinant adeno-associated virus vaccine combinatorial immunization specific cell-mediated immune responses 

摘      要：Cellular immune responses,particularly those associated with CD3+CD8+ cytotoxic T lymphocytes (CTL),are critical factors in controlling viral *** carcinoma (NPC) is closely associated with persistent Epstein-Barr virus (EBV) *** vaccine studies have focused on enhancing specific antiviral CTL *** this study,three vaccines capable of expressing the EBV-latent membrane protein 2 (LMP2) (a DNA vector,an adeno-associated virus (AAV) vector,and a replication-defective adenovirus serotype 5 (Ad5) vector) were respectively used to immunize female Balb/c mice (4-6 weeks old) at weeks 0,2 and 4,either alone or in *** results suggest that combined immunization with DNA,AAV,and adenovirus vector vaccines induced specific cellular immunity more effectively than any of these vectors alone or a combination of two of the three,constituting a sound vaccine strategy for the prevention and treatment of NPC.