Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet

作     者：Yanqing Guan Zhibin Li Xin Wang Xiaoli Ni Aini Yang Junming Liu 

作者机构：School of Life Science South China Normal University Guangzhou 510631 China School of Physics South China Normal University Guangzhou 510631 China Laboratory of Solid State Microstructures Nanjing University Nanjing 210093 China International Center for Materials Physics Chinese Academy of Sciences Shenyang 110016 China 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2010年第26卷第12期

页      面：1119-1126页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0703[理学-化学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China (Nos.30970731 and 50832002) the Natural Science Foundation of Guangdong Province(No.9151063101000015) 

主　　题：N-isopropylacrylamide Low critical solution temperature Tissue engineering Corneal epithelial cells 

摘      要：In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p（NIPAAm-co-AA）, which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography （HPLC）, Fourier transform infrared （FTIR） and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature （LCST） testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate （PSt） was measured by electron spectroscopy for chemical analysis （ESCA）. The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.