Expressional regulation in vitro of psbA mRNA 3' untranslated region in rice and sorghum

作     者：钟珍萍 吴乃虎 

作者机构：Institute of Developmental Biology Chinese Academy of Sciences Beijing 100080 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：1997年第40卷第4期

页      面：406-413页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0828[工学-农业工程] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Project supported by the Chinese National High-Tech Project 

主　　题：rice sorghum psbA 3’UTR stem-loop IR sequence regulation. 

摘      要：To study the relationship between the structures of rice and sorghum psbA 3 UTR and gene expres-sional activity, six chimeric luc genes that encode luciferase under control of rice or sorghum psbA 5 UTR and 3’UTR or only 5 UTR were constructed. The levels of LUC accumulation in E . coli and the transcript stability in soluble pro-tein extracts of rice, sorghum chloroplast and E. coli were examined respectively, and a detailed analysis about the function of these two 3’UTR has been carried out. Here the regulation effect of these 3’UTR are reported: ( i ) When having the same promoter, the chimeric genes with rice 3 UTR produce LUC much more than that with sorghum 3 UTR; ( ii ) elimination of 3 UTR results in the fluctuation of LUC accumulation no matter whether it is under control of rice or sorghum psbA 5’UTR; ( Hi ) rice psbA 3’UTR exhibits a greater effect on stabilizing tran-scripts; ( IV ) psbA 3’lR-RNAs are more stable in chloroplast protein extracts than in E. coli protein extracts.