Platelet aggregation is affected by nitrosothiols in patients with chronic hepatitis: In vivo and in vitro studies

作     者：A Federico A Filippelli M Falciani C Tuccillo A Tiso A Floreani R Naccarato F Rossi C Del Vecchio Blanco C Loguercio 

作者机构：Inter-University Research Centre on Foods Nutrition and Gastrointestinal Tract Gastroenterology School 2nd University of Naples Italy Department of Experimental Medicine 2nd University of Naples Italy Department of Surgical and Gastroenterological Sciences University of Padua Italy 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2007年第13卷第27期

页      面：3677-3683页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：肝病 血小板功能 慢性肝炎 氧化应急 

摘      要：AIM: To investigate the relationship among the number of platelets and plasma levels of S-nitrosothiols (S-NO), nitrite, total non-protein SH (NPSH), glutathione (GSH), cysteine (CYS), malondialdehyde (MDA), 4-hydroxininenal (4HNE), tumor necrosis factor-alpha (TNFα) and interleukin (IL)-6 in patients with chronic hepatitis C (CH).METHODS: In vitro the aggregation of platelets derived from controls and CH patients was evaluated before and after the addition of adenosine diphosphate (ADP) and collagen, both in basal conditions and after incubation with nitrosoglutathione (GSNO). RESULTS: In vivo , S-NO plasma levels increased significantly in CH patients and they were significantly directly correlated with platelet numbers. Patients with platelet counts 150 000/μL. In vitro, the ADP and collagen aggregation time was increased in platelets from patients and not from controls; in addition, platelets from CH patients but not from controls also showed a latency time after exposure to collagen. CONCLUSION: The incubation of platelets with GSNO improved the percentage aggregation and abolished the latency time.