Characteristics of global cerebral ischemia models constructed by modified four-vessel occlusion in rats

作     者：Jinbao Li Lai Jiang Hua Xu Yuanchang Xiong Xiaoming Deng 

作者机构：Department of Anesthesiology Changhai Hospital Second Military Medical University of ChinesePLA Shanghai 200433 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2006年第1卷第5期

页      面：436-440页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

主　　题：cerebral ischemia blood pressure rectal temperature arterial blood gas dentate gyrus common carotid artery pathological change Experimental Animal rat model carotid arteries result analysis modeling method blood flow 

摘      要：BACKGROUND： Pulsinelli et al developed a kind of rat models with four-vessel occlusion-induced global cerebral ischemia, Because the histo-pathological changes and severe cerebral ischemia reproducibility of this model are good and the stability of this model in circulation respiration is superior to that of other models, so four-vessel occlusion method has become a classic modeling method for global cerebral ischemia model, This model has been improved in some laboratories to meet different requirements in different studies. OBJECTIVE： To establish a highly reproducible rat model of reversible forebrain ischemia by modifying four-vessel occlusion model introduced by Pulsinelli et al, and to investigate its neurophysiological and pathological changes and the characteristics of modified operation. DESIGN ： Completely randomized grouping, controlled tria. SETTING ： Department of Anesthesiology,Changhai Hospital, Second Military Medical University of Chinese PLA. MATERIALS ： A total of 65 male healthy SD rats, weighing 250-300 g, were provided by the Experimental Animal Center of Second Military Medical University of Chinese PLA. VSM hemodynamic monitor and temperature monitor （Thermal ert TH-5, U.S.A） were used. METHODS ： The trial was conducted in the Department of Anesthesiology, Changhai Hospital, Second Military Medical University of Chinese PLA from January 2005 to March 2006. ① Experimental grouping： Sixty-five rats were randomly divided into the following 7 groups： sham-operation group （n =9）： given the same operation, without occlusion of vessels; 5 minutes ischemia group （n =9）： ischemia 5 minutes and reperfusion 72 hours; 10 minutes ischemia group （n =8）： ischemia 10 minutes and reperfusion 72 hours; 15 minutes ischemia group （n =9）： ischemia 15 minutes and reperfusion 72 hours; 20 minutes ischemia group （n =8）： ischemia 20 minutes and reperfusion 72 hours; 30 minutes ischemia group （n =7）; ischemia 30 minutes and reperfusion 72 hours; ischemia con