Study on Alkaloids in Fructus Hordei Germinatus

作     者：Jianheng GUO Guozhu LI Qingyan MENG Jizhong ZHANG 

作者机构：School of PharmacySouthwest Minzu University Alar First Division of Institute for Food and Drug Control Key Laboratory of Resource Protection and Utilization of Tarim BasinXinjiang Production & Construction Ethnic Medicine InstituteSouthwest Minzu University 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2018年第9卷第6期

页      面：19-22页

学科分类：10[医学] 

基　　金：Supported by Innovating Research Program of Postgraduates of Southwest Minzu University in 2017(CX2017SZ086) 

主　　题：Fructus Hordei Germinatus Alkaloids Ultra high performance liquid chromatography(UPLC) Mass spectrometer(MS) Preparative high performance liquid chromatography(pHPLC) 

摘      要：[Objectives]To isolate and analyze alkaloids in Fructus Hordei Germinatus.[Methods]The alkaloids in the Fructus Hordei Germinatus were extracted by ultrasonic technology,and analyzed by the ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) technique.[Results] According to the mass spectrometry information,compared with the literature reports,the possible structures of seven alkaloids in the Fructus Hordei Germinatus could be *** 1,2 and 3 were organic amine alkaloids,respectively tyramine and N-methyltyramine,hordenine,compounds 4,5,6 and 7 were indole alkaloids,respectively,tryptamine,gramine,harmine,and *** alkaloids in the Fructus Hordei Germinatus were separated by preparative high performance liquid chromatography(p HPLC) technique,a compound was obtained and identified by UPLC-MS/MS and nuclear magnetic resonance(NMR) technique as hordenine.[Conclusions]This experiment is expected to lay a foundation for the development of alkaloids in the Fructus Hordei Germinatus.