Apoptosis and proliferation of intrahepatic bil educt after ischemia-reperfusion injury

作     者：Wen-Hui Xu, Qi-Fa Ye and Sui-Sheng Xia Institute of Organ Transplantation, Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China 

作者机构：Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. jzxwh@*** 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2004年第3卷第3期

页      面：428-432页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：apoptosis proliferation intrahepatic bile duct epithelial cells 

摘      要：BACKGROUND: In orthotopic liver transplantation, ische-mic-reperfusion is one of the most important factors thatcause the incidence of biliary complicance. The aim of thisstudy was to investigate the effects of ischemia reperfusionon epithelial cells apoptosis and proliferation of intrahepaticbile duct (IBD) (20 μm).METHODS: 30-minute warm ischemia was applied to ratlivers respectively, and experiment was performed on days2,7, 14, 28 after reperfusion. Apoptosis was determined insitu by morphology and TUNEL, and cholangiocyte proli-feration was evaluated in situ by morphometry of liver sec-tions stained for cytokeratin-19 ( CK-19) and by prolifera-ting cellular nuclear antigen staining in liver ***: Two days after ischemia reperfusion, apoptosisof cells was observed in large intrahepatic bile ducts (20μm) (5.6%±1.2%) , but the number of large intrahepaticbile ducts reduced (0.32 ±0.06). Seven days after ischemiareperfusion, the apoptosis index of cholangiocytes de-creased to 1.2%±0.3%, and the number of intrahepatic bileducts began to proliferate and returned to nearly normal onday ***: Ischemia reperfusion causes a decrease inthe number of intrahepatic bile ducts (20 μm) as a resultof a higher rate of apoptosis and absence of initial prolifera-tion.