Shenfu Injection(参附注射液) Suppresses Inflammation by Targeting Haptoglobin and Pentraxin 3 in Rats with Chronic Ischemic Heart Failure

作     者：郑思道 吴红金 余少平 任建勋 多韦唯 马增春 高月 王升启 刘宇娜 

作者机构：Beijing Hospital of Integrated Traditional Chinese and Western Medicine Beijing Institute of Radiation Medicine 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2015年第21卷第1期

页      面：22-28页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Science and Technology Fund of the Beijing Bureau of Traditional Chinese Medicine(No.JJ2001-37) the National Nature Science Foundation of China(No.81173367) 

主　　题：Shenfu Injection chronic heart failure proteomics haptoglobin pentraxin 3 

摘      要：Objective： To investigate the regulatory effects of Shenfu Injection （SFI, 参附注射液） on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure （CHF）. Methods： Forty-five healthy Wistar rats were randomized into three groups： sham, heart failure （model） and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation, animals in the sham group and the model group received saline （6.2 mL/kg/d）, while animals in the SFI group received SFI （6.2 mL/kg.d） intraperitoneally. Four weeks later, cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrixassisted laser desorption/ionization time-of-flight mass spectrometer （MALDI-TOF MS）. Results： Recording of hemodynamic parameters showed that left ventricular systolic pressure （LVSP）, maximum rate of left ventricular pressure （＋dp/dtmax） rise, and maximum rate of left ventricular pressure （-dp/dtmax） decrease, while the left ventricular end diastolic pressure （LVEDP） rose in the model group compared to those in the sham group （P〈0.05）. The results of the MALDI-TOF MS indicated that haptoglobin （HP）, pentraxin 3 （PTX3） and alpha-1- antitrypsin were up-regulated, while serum albumin and 40S ribosomal protein were down-regulated in the model group （P〈0.05）. Compared with the model group, LVSP, ＋dp/dtmax and -dp/dtmax were higher, while LVEDP was lower in the SFI group （P〈0.05）. Expression levels of HP and PTX3 were lower than in the model group （P〈0.05）. Conclusion： SFI could improve hemodynamic function and decrease inflammatory reactions in the pathophysiology of CHF. The serum proteins HP and PTX3 could be potential biomarkers for chronic ischemic heart failure, and they could also be the serum protein targets of SFI.