Antitumor effect of cytosine deaminase/5-fluorocytosine suicide gene therapy system mediated by Bifidobacterium infantis on melanoma

作     者：Cheng YI~2,Ying HUANG~3,Zhi-ying GUO~3,Shu-ren WANG~(3,4) ~2Cancer Center,West China Hospital,Sichuan University Chengdu,610041,China ~3Department of Pathophysiology,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China 

作者机构：CancerCenterWestChinaHospitalSichuanUniversityChengdu610041China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2005年第26卷第5期

页      面：629-634页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by CMB project(MERF-2002) 

主　　题：Bifidobacterium infantis cytosine deaminase gene therapy 

摘      要：Aim:To construct a Bifidobacterium infantis/CD targeting gene therapy systemand observe the antitumor effect of cytosine deaminase/5-fluorocytosine(CD/5-FC)suicide gene therapy system mediated by Bifidobacterium infantis on mela-noma in vitro and in ***:A recombinant CD/pGEX-1LamdaT plasmidwas transfected into Bifidobacterium infantis by ***-terium infantis transfected by recombinant CD/pGEX-1LamdaT plasmid was in-cubated with 5-FC *** the supernatant fluid was collected andadded to melanoma B 16-F10 cells to observe the killing effect for B 16-F10 *** were inoculated with melanoma B 16-F10 cells to establish animal *** mice were then injected with 5-FC and Bifidobacterium infantis transfectedby recombinant CD/pGEX-1LamdaT ***:Two segments of approxi-mate 4.9 kb and 1.3 kb were extracted from the 6.2 kb recombinant plasmid,whichwere equal to the size of the pGEX-1LamdaT plasmid and CD gene,*** results showed that the full length and sequence of nucleotide acid ofthe inserted gene in extracted recombinant plasmid was completely identical to theCD *** vitro,B16-F10 cells treated by supernatant fluid were remarkablydamaged morphologically,and the cell growth was significantly ***-ments on the mice melanoma model showed that after treatment with a combina-tion of transfected Bifidobacterium infantis and 5-FC,the tumor volume wassignificantly inhibited compared with ***:The foreign gene,CD gene,was correctly inserted into pGEX-1LambdaT plasmid and transferredinto Bifidobacterium ***/5-FC suicide gene therapy system mediated byBifidobacterium infantis demonstrated a good antitumor effect on melanoma invitro and in vivo.