Molecular Cloning and Functional Analysis of ESGP,an Embryonic Stem Cell and Germ Cell Specific Protein
Molecular Cloning and Functional Analysis of ESGP,an Embryonic Stem Cell and Germ Cell Specific Protein作者机构：Laboratory of Molecular and Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China
出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))
年 卷 期：2005年第37卷第12期
页 面：7+790-796页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 071009[理学-细胞生物学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
主 题：embryonic stem cell and germ cell specific protein(ESGP)gene embryonic stem cell Oct-4 self-renewal
摘 要：Several putative Oct-4 downstream genes from mouse embryonic stem (ES) cells have beenidentified using the suppression-subtractive hybridization *** this study,one of the novel genesencoding an ES cell and germ cell specific protein (ESGP) was cloned by rapid amplification of cDNA *** contains 801 bp encoding an 84 amino acid small protein and has no significant homology to anyknown *** is a signal peptide at the N-terminal of ESGP protein as predicted by SeqWeb (GCG)(SeqWeb version 2.0.2,http://***:8080/).The result of immunofluorescence assay suggestedthat ESGP might encode a secretory *** expression pattern of ESGP is consistent with the expres-sion of Oct-4 during embryonic *** protein was detected in fertilized oocyte,from 3.5 daypostcoital (dpc) blastocyst to 17.5 dpc embryo,and was only detected in testis and ovary tissues in ***,ESGP was only expressed in pluripotent cell lines,such as embryonic stem cells,embryonic carcinomacells and embryonic germ cells,but not in their differentiated *** its specific expression,forced expression of ESGP is not indispensable for the effect of Oct-4 on ES cell self-renewal,and does notaffect the differentiation to three germ layers.
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