Cloning and Sequence of Glycoprotein H Gene of Duck Plague Virus

作     者：HAN Xian-jie WANG Jun-wei MA Bo 

作者机构：Faculty of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R. China Faculty of Animal Science and Veterinary Medicine Laiyang Agricultural College Qingdao 266109 P.R. China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2006年第5卷第5期

页      面：397-402页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

主　　题：duck plague virus glycoprotein H gene degenerate PCR 

摘      要：The glycoprotein H （gH） gene homologue of duck plague virus （DPV） was cloned by degenerate polymerase chain reaction （PCR） and sequenced. It was located immediately downstream from the thymidine kinase gene （TK）. In addition, the 3＇-end of the gene homologue to herpesvirus UL21 was located downstream from the gH gene. DPV gH gene open reading frame （ORF） was 2 505 bp in length and its primary translation product was a polypeptide of 834 amino acids long. It possessed several characteristics of membrane glycoproteins, including an N-terminal hydrophobic signal sequence, an external domain containing eight putative N-linked glycosylation sites, a C-terminal transmembrane domain, and a charged cytoplasmic tail. Comparison with other herpesvirus revealed identities of 20.2, 25.1, 23.0, 23.0, 26.5 and 26.0% with the gH counterparts of the human herpesvirus virus 1 （HSV1）, equine herpesvirus 4 （EHV4）, bovine herpesvirus 1 （BHV1）, pseudorabies virus （PRV）, gallid herpesvirus 2 （GHV2） and gallid herpesvirus 3 （GHV3）, respectively.