DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters

作     者：Qi-lian LIANG Bi-rong WANG Guo-hong LI 

作者机构：Department of Medical Oncology Affiliated Hospital of Guangdong Medical College Zhanjiang 524001 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2009年第10卷第9期

页      面：675-682页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：Death decoy receptor 3 (DcR3) Survivin Colorectal carcinoma 

摘      要：Objective: To investigate the expression of death decoy receptor 3 (DcR3) and survivin in colorectal carcinoma. Methods: Tumor and normal tissues were taken from a total of 100 colorectal carcinoma patients during surgery, and the expression of DcR3 and survivin was examined by immunohistochemistry, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) analyses. Results: RT-PCR showed that the expression levels of DcR3 mRNA (0.846±0.242, P0.01) and survivin mRNA (0.7835±0.2392, P0.01) in colorectal cancer tissues were significantly higher than those in adjacent normal tissues. Western blotting showed that the expression levels of DcR3 protein (0.795±0.261, P0.01) and survivin protein (0.6765±0.1351, P0.01) in tumor tissues were significantly higher than those in non-cancer tissues. The immunohistochemical streptavidin-peroxidase (SP) method showed that the positive expression rates of DcR3 and survivin were 67.0% and 58.0% in colorectal cancer tissues, and 18.0% and 3.0% in non-cancerous colorectal tissues (P0.05), respectively. The positive correlations of DcR3 (P0.01) and survivin (P0.01) to the differentiation of colorectal carcinoma cells, lymph node metastasis, and patho-logical stage were observed. The expression of DcR3 and survivin was found to be positively correlated to clinicopathologic parameters of colorectal carcinoma. Conclusion: The overexpressed DcR3 and survivin in colorectal cancer may contribute to the development of the cancer. The monitoring of these two proteins may be useful for the diagnosis, differentiation, metastasis, and determination of stages of colorectal carcinoma.