San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice

作     者：WANG Min SUN Yan ZHANG Shu-jing GAO Yu-shan WEI Ya-nan ZHAO Peng-na LIU Miao YANG Jia-min ZHENG Feng-jie XU Hong LI Yu-hang WANG Min;SUN Yan;ZHANG Shu-jing;GAO Yu-shan;WEI Ya-nan;ZHAO Peng-na;LIU Miao;YANG Jia-min;ZHENG Feng-jie;XU Hong;LI Yu-hang

作者机构：School of Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2021年第27卷第3期

页      面：212-219页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation(No.81373503) 

主　　题：regulation of fluid passage urine volume aquaporin San-Ao Decoction bronchial asthma 

摘      要：Objective To observe the effect of San-Ao Decoction(三拗汤,SAD)on water metabolism of bronchial asthra model *** Forty-five female BALB/c mice were randomly divided into control,model and SAD groups by a random number table,15 mice in each group.A composite method with ovalbumin(OVA)sensitization and challenge was developed to establish bronchial asthma *** in the control group were intraperitoneally injected with distilled water without aerosol inhalation *** day 15–22,0.3 mL SAD was administered via gastric route in SAD group,one time per day,while an equivalent volume of normal saline was used for gastric administration in the control and model *** in airway resistance in the inspiratory phase(RI-R-Area)were detected using an AniRes2005 system,and 5-h urine output was collected by metabolic *** changes in lung and kidney were observed by hematoxylin-eosin *** expressions of aquaporin(AQP)1 and AQP2 in kidney were detected by reverse transcription-polymerase chain reaction,and the protein expressions of AQP1 and AQP2 in kidney were detected by ***-linked immune sorbent assay was used to detect the OVA-specific endothelium-1(ET-1),antidiuretic hormone(ADH),atrial natriuretic peptide(ANP),prostaglandin E_(2)(PGE_(2)),and angiotensin II(Ang II)levels in serum,lung and kidney tissues,*** nitric oxide(NO)contents in serum,lung,and kidney tissues were tested by chemical method,*** Compared with the control group,the serum IgE level in model group increased(P0.01).Following the pathologic changes in lung tissue,no significant change in kidney tissue was observed among 3 *** with the control group,the mice in the model group showed elevated airway resistance during inhalation phase,higher mRNA and protein expression levels on AQP1 and AQP2 in kidney tissue and higher ET-1 levels in serum,lung and kidney tissues,ADH and ANP in lung and serum,PGE