Simple and effective detection method for gene expression product of transgenic plant

作     者：Jiaxi Xu Mi Ma Yuan Ma Zhongping Lin 

作者机构：Peking Univ Coll Chem & Mol Engn Beijing 100871 Peoples R China Chinese Acad Sci Inst Bot Beijing 100093 Peoples R China Tsing Hua Univ Dept Chem Bioorgan Phosphorus Chem Lab Beijing 100864 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：1999年第44卷第10期

页      面：912-916页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：National Natural Science Foundation of China, NSFC, (29572034, 29772002, 39470158) National Natural Science Foundation of China, NSFC 

主　　题：transgenic plant isopentenyl transferase antigenic peptide detective method. 

摘      要：It is a crucial problem in gene engineering whether an exogenous gene could be correctly expressed in cells of transgenic animal or plant, and how the expression products could be detected quantitatively in translational level. It is very difficult to analyze some gene expression products, such as isopentenyl transferase (ipt), in transgenic investigation because they are trace in organisms. A convenient method is described to determine trace content gene expression product which is hard to purify. The method includes predicting and synthesizing the antigenic peptide according to the cDNA sequence, coupling the synthetic antigenic peptide with carrier protein, raising specific antibodies against the synthetic antigen, and detecting the gene expression product by ELISA and Western blot.