Effects of acupoint versus non-acupoint electroacupuncture on cerebral cortical neuronal Bcl-2, Bax and caspase-3 expression in a rat model of focal cerebral ischemia

作     者：Jun Wang Junming Fan Yongshu Dong Xia Huang Hongxia Zhang 

作者机构：Laboratory of Pharmacology Henan Institute of Traditional Chinese Medicine Zhengzhou 450004 Henan Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2008年第3卷第12期

页      面：1308-1313页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：the National Natural Science Foundation of China, No. 39270837 the Natural Science Foundation of Henan Province, No. 984021000 

主　　题：electroacupuncture cerebral ischemia/reperfusion apoptosis caspase-3 Bcl-2 Bax 

摘      要：BACKGROUND： Several studies have demonstrated that electroacupuncture by acupoint selection can inhibit cerebral cortical neuronal apoptosis following cerebral ischemia/reperfusion. OBJECTIVE： To validate the effects of electroacupuncture by acupoint selection on the expression level of cortical neuronal anti-apoptotic Bcl-2 protein and the apoptotic executive protein, caspase-3, in rat models of focal cerebral ischemia/reperfusion. DESIGN, TIME AND SETTING： This randomized grouping, neural cell and molecular biology animal experiment was performed at the Laboratory of Pharmacology of Traditional Chinese Medicine and the Laboratory Animal Center of Henan Institute of Traditional Chinese Medicine between November 2006 and May 2007. MATERIALS： Atotal of 40 healthy male adult Sprague-Dawley rats were randomly and evenly divided into four groups： sham-operated, model, electroacupuncture and non-aeupoint control. G6895 electro-acupuncture instruments were purchased from Shanghai Huayi Instrument Factory, China. Caspase-3, Bcl-2 and Bax kits were provided by Wuhan Boster Bioengineering Co., Ltd., China. METHODS： Middle cerebral artery occlusion was induced in the model, electroacupuncture and non-acupoint groups. In the electroacupuncture group, the acupoints Jianyu （LII5）, Waiguan （S J5）, Biguan （ST31）, and Zusanli （ST36） were given electroacupuncture. In the non-acupoint control group, at each time point （immediately after ischemia and after reperfusion, or 2 hours after reperfusion）, electroacupuncture was performed at the midpoints of Tianquan （PC2）-Quze （PC 3） line, Quze （PC 3）-Ximen （PC4） line, Zuwuli （LR10）-Yinbao （LR9） line, and Xiguan （LR7）-Zhongdu （LR6） line. Electroacupuncture parameters were set with a continuous wave with a frequency of 10 Hz, wave width 0.6 ms, voltage 1.5-3.0 V, and a duration of 10 minutes. The sham-operated and model groups received only animal fixation without electroacupuncture procedure. MAIN OUTCOME MEASURES： Five rats were selected f