Cell sheet therapy for myocardial infarction

作     者：Dehua Chang Tatsuya Shimizu Teruo Okano 

作者机构：Institute of Advanced Biomedical Engineering and ScienceTokyo Women's Medical University 

出 版 物：《岭南心血管病杂志》 (South China Journal of Cardiovascular Diseases)

年 卷 期：2011年第17卷第S1期

页      面：23-24页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：sheet descending myocardium anesthesia transplant responsive multilayer overcome powerful created 

摘      要：Introduction Recently,bone marrow mesenchymal stem cells(MSCs) have been reported to repair chronically infracted myocardium with direct ***,it is very difficult to localize the injected cells onto the ischemic area to regenerate sufficient cardiac mass in the thinned scar *** overcome the problem,we have utilized our cell sheet technology based on temperature-responsive culture *** the culture temperature is reduced from 37℃to 20℃,all cells connected via cell-cell junction proteins are harvested as a single sheet without using proteolytic *** technology allows us to transplant stem cells in vivo for treatment heart disease without the problems mention in the *** Male Clawn mini pigs were used in this *** marrow(5-7 mL)was collected under general anesthesia. Histopaqe-1077(15 mL),were added to bone marrow and *** cells were collected and cultured for 7 *** seeded the bone marrow-derived MSCs at the concentration of(6×10~5/ml) on 60 mm diameter temperature -responsive dishes for 7 *** the culture temperature decreased from 37℃to 20℃,MSC sheet detached itself spon-taneously and floated up into the culture medium. Triple layers were stacked together repeatedly forming special *** infarction was created by the ligation of the left anterior descending branch of the left coronary artery.A cell sheets was transplanted onto the ischemia *** echocardiography was performed two and four weeks after *** heart tissue with cell sheets were removed and fixed with 10%formalin for histological analysis one month after the transplantation of cell *** Most MSCs are positive for CD29,CD90,CD146 and *** mean the culture cell sheets were composed of undifferentiated MSCs and remained ***(20- 30μm) and multilayer(120μm) cell sheets were produced,which retained all cell-to-cell contaction. Histological analyses show the cell sheets become c