Differentially Expressed Genes of Soybean During Infection by Phytophthora sojae

作     者：XU Peng-fei WU Jun-jiang Allen Xue LI Wen-bin CHEN Wei-yuan WEI Lai LV Hui-ying LIN Shi-feng FAN Su-jie LI Ning-hui WANG Xin JIANG Liang-yu ZHANG Shu-zhen 

作者机构：Key Laboratory of Soybean BiologyMinistry of Education/Soybean Research InstituteNortheast Agricultural UniversityHarbin 150030P.R.China Soybean Research InstituteHeilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOntario K1A 0C6Canada Suihua Research InstituteHeilongjiang Academy of Agricultural SciencesSuihua 152052P.R.China Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101P.R.China College of Agriculture and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第3期

页      面：368-377页

核心收录：

学科分类：07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：supported by the Program for New Century Excellent Talents in Universities,Ministry of Education,China(NCET-09-164) the National Natural Science Foundation of China(30671317,30971811,31071439,and 31110103001) the Program for New Century Excellent Talents in Universities in Heilongjiang Province,China(NCET-06-007) the Natural Science Foundation of Heilongjiang Province,China(C200814) 

主　　题：cDNA microarray Glycine max Phytophthora sojae soybean suppressed subtraction hybridization 

摘      要：To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae,a cDNA library for suppression subtractive hybridization （SSH） was constructed with cDNAs from soybean cultivar Suinong 10 treated with sterile distilled water as the driver and cDNAs from Suinong 10 inoculated with *** as the tester.A total of 2 067 recombinant colonies from the SSH library were randomly picked,amplified,and *** discarding 312 poor quality expressed sequence tags （EST）,1 755 high quality ESTs were assembled and edited to 1 384 tentatively unique genes （TUG）,in which,586 showed significant homology to known sequences,and 798 had low homology or no match with the known sequences.A cDNA microarray containing 307 singletons from the 586 TUGs and 222 singletons from the 798 TUGs was developed to characterize differentially expressed cDNAs in the SSH library,and eight cDNAs were identified to be up-regulated after microarray analysis and then confirmed by real-time *** were homologous to the protein 10,and were also related to some proteins in disease resistance response,such as pathogen-related protein,phenylalanine ammonia-lyase,isoflavone reductase,WRKY transcription factor 31,major allergen Pru ar 1,and pleiotropic drug resistance protein *** of the up-regulated cDNAs encode enzymes of phytoalexin biosynthesis and pathogenesis-related proteins involved in plant disease ***,we fist reported the Pru ar 1 in *** findings of this research have contributed to better understanding of soybean resistance to *** at the molecular level.