Resistin does not down-regulate the transcription of insulin receptor promoter
Resistin does not down-regulate the transcription of insulin receptor promoter作者机构:Department of Very Important Person the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Institute of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China
出 版 物:《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报(英文版)B辑(生物医学与生物技术))
年 卷 期:2008年第9卷第4期
页 面:313-318页
核心收录:
学科分类:1002[医学-临床医学] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金:Project supported by the National 11th Five-Year Plan of Scientific and Technological Program (No. 2006BAI02B08) of China theDepartment of Science and Technology of Zhejiang Province (No. 2003C33031), China
主 题:Resistin Insulin receptor promoter Luciferase reporter gene
摘 要:Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.