Hessian single-molecule localization microscopy using sCMOS camera

作     者：Fudong Xue Wenting He Fan Xu Mingshu Zhang Liangyi Chen Pingyong Xu 

作者机构：Key Laboratory of RNA Biology Institute of Biophysics Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Weldon School of Biomedical Engineering Purdue University State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular Medicine Peking University 

出 版 物：《Biophysics Reports》 (生物物理学报(英文))

年 卷 期：2018年第4卷第4期

页      面：215-221页

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

基　　金：supported by the National Key R&D Program of China (2016YFA0501500 and 2017YFA0505300) the National Natural Science Foundation of China (31421002, 21778069 and 31670870) Project of the Chinese Academy of Sciences (XDB08030203) CAS-Peking University Joint Team Project 

主　　题：Super-resolution Hessian SMLM sCMOS 

摘      要：Single-molecule localization microscopy(SMLM) has the highest spatial resolution among the existing super-resolution imaging techniques, but its temporal resolution needs further improvement. An s CMOS camera can effectively increase the imaging rate due to its large field of view and fast imaging speed. Using an s CMOS camera for SMLM imaging can significantly improve the imaging time resolution, but the unique single-pixel-dependent readout noise of s CMOS cameras severely limits their application in SMLM imaging. This paper develops a Hessian-based SMLM(Hessian-SMLM) method that can correct the variance, gain, and offset of a single pixel of a camera and effectively eliminate the pixeldependent readout noise of s CMOS cameras, especially when the signal-to-noise ratio is low. Using Hessian-SMLM to image mEos3.2-labeled actin was able to significantly reduce the artifacts due to camera noise.