使用激光捕获显微切制法对黑色素患者及其配偶的黑素细胞痣CDKN2A/p16INK4a突变的研究
A search for CDKN2A/p16INK4a mutations in melanocytic nevi from patients with melanoma and spouse controls by use of laser-captured microdissection作者机构：Dr.Division of Dermatology Box 356524 University of Washington Seattle WA 98195 United States
出 版 物：《世界核心医学期刊文摘（皮肤病学分册）》 (Digest of the World Core Medical JOurnals:Dermatology)
年 卷 期：2005年第1卷第6期
页 面：49-50页
学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学]
主 题：黑素细胞痣 黑色素瘤 显微解剖 点突变 编码区 组织发生 多效
摘 要：Objective: To determine the frequency at which the CDKN2A coding region is mutated in the atypical nevi of persons with sporadic melanoma. Design: DNA samples, isolated by laser-captured microdissection of atypical nevi from 10 patients with newly incident cases of sporadic melanoma and their spouses as matched controls, were used as templates for nested polymerase chain reaction amplification of CDKN2A exons 1 and 2. Results: No point mutations in the coding region of CDKN2A were observed in any of the melanocytic nevi. Conclusions: Point mutations in CDKN2A are an uncommon event in the atypical nevi of persons with melanoma. As such, the data may support a hypothesis of melanocytic nevus histogenesis, in which the melanocytic nevus and malignant melanoma represent separate, pleiotropic pathways resulting from common stimuli, such as genomic damage from UV radiation.
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