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Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines

Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines

作     者:蔡宏 田霞 呼西旦 潘怡 李国利 庄玉辉 朱玉贤 

作者机构:China Peking University Tuberculosis Research Laboratory 309 Hospital of PLA Institute of Veterinary Xinjiang Academy of Animal Science Beijing 100091 Peking University Beijing 100871 College of Life Science National Laboratory of Protein Engineering and Plant Genetic Engineering Urumqi 830000 

出 版 物:《Science China Life Sciences》 (中国科学:生命科学(英文版))

年 卷 期:2003年第46卷第5期

页      面:495-502+562页

核心收录:

学科分类:1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基  金:Chinese National High-Tech 863 Program 

主  题:Mycobacterium tuberculosis secreted proteins combination DNA vaccines immunogenicity protective efficacy. 

摘      要:Immune response and protective efficacy for the combination of four tuberculosis DNA vaccines were evaluated in this study. We obtained 1︰200 antibody titers against Ag85B 21d after mice were vaccinated for the first time by four recombinant eukaryotic expression vectors containing coding sequences for Ag85B, MPT-64, MPT-63 and ESAT-6. The titers of Ag85B were elevated to 1︰102400 after the second injection and decreased to 1︰12800 after the third injection. Antibody titers for MPT-64 and MPT-63 reached 1︰25600 21 d after the first vaccination, and were then decreased following the second and third injections. No antigen-specific antibody titer against ESAT-6 was detected in sera harvested from immunized mice at any time. These DNA vaccines evoked specific IFN-g responses in the spleens of vaccinated mice as well. When challenged with M. tuberculosis H37Rv, we found that the lungs of the vaccinated mice produced 99.8% less bacterial counts than that of the empty-vector control group and the bacterial counts were also significantly less than that of the BCG group. Histopathological analyses showed that the lungs of vaccinated mice produced no obvious caseation while over 50%—70% of the pulmonary parenchyma tissue produced central caseation in the vector control group. Our results indicated that the combination of four tuberculosis DNA vaccines may generate high levels of immune responses and result in better animal protection.

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