THE EFFECT OF TISSUE DISAGGREGATION METHODS ON THE CLONOGENICITY OF SOLID HUMAN TUMOR CELLS

作     者：李同光 Lisa D Blackburn Ronnie D Horner Jan F Silverman Albert L Wiley Jr. 

作者机构：Departments of Radiation Oncology Family Medicine Clinical Pathology and Diagnostic Medicine East Carolina University School of Medicine Greenville NC 27858 USA 

出 版 物：《中华医学杂志(英文版)》 (Chinese Medical Journal)

年 卷 期：1992年第105卷第2期

页      面：126-131页

核心收录：

学科分类：10[医学] 

主　　题：SOFT-AGAR GROWTH ASSAY 

摘      要：The effect of mechanical,enzymatic and com-bined disaggregations on the same tumor tissue(n=154)to define variables related to clonogenic effi-ciency (CE) of human tumor clonogenic assay(HTCA)was ***,CE was highly asso-ciated with the percentage of malignant cells in theinoculative suspensions(P0.001)and the total cellconcentration plated(P=0.03).However,there was nosignificant correlation between CE and thedisaggregation method,type of tumor,cell viability,proportion of macrophages in the inoculum,or lengthof incubation(10 or 20 days).In addition,a higher CEwas found in some nonadherent fractions when com-paring the CE of the original plating suspensions tothat of their nonadherent *** is concluded thatmechanical disaggregation is the simplest technique forobtaining the highest yield of malignant cells,which isa decisive factor for colony growth,and that anincubation time of 10 days and 1×10～6cells per plate is the best condition for the human tu-mor clonogenic assay.