Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using （-）-gossypol
左旋棉酚对前列腺癌PC-3细胞增殖的体内外抑制作用作者机构：The Key Laboratory of Biomedical Information Engineering of Ministry of Education Xi ＇an Jiaotong University Xi ＇an 710038 China Department of Blood Transfusion Xij＇ing Hospital Xi ＇an 710032 China Central Laboratory Fourth Military Medical University Xi＇an 710032 China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2010年第12卷第3期
页 面：390-399,I0011页
核心收录：
基 金：This study was supported in part by grants from National Natural Science Foundation of China (No. 30570494 and No. 30772658). We thank Dr Xing- Bin Hu (The Second Department of Blood Transfusion Xijing Hospital Xi'an China) for assisting writing this manuscript
主 题：apoptosis electron microscopy flow cytometry (-)-gossypol immunohistochemistry prostate cancer
摘 要：We investigated the antiproliferative activity of （-）-gossypol on the human prostate cancer cell line PC3 in vitro and in vivo to elucidate its potential molecular mechanisms. Cell growth and viability were evaluated using the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay, and cell apoptosis was detected by flow cytometry, terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） and electron microscopy. Expression of proliferating cell nuclear antigen （PCNA）, Bcl-2, CD31, caspase-3 and caspase-8 in tumour tissue was determined by immunohistochemistry. The drug concentration that yielded 50% cell inhibition （IC50 value） was 4.74 μg mL-1. In the PC-3 tumour xenograft study, （-）-gossypol （〉 5 mg kg-1） given once a day for 7 days significantly inhibited tumour growth in a dose-dependent manner. Immunohistochemical analysis revealed that （-）-gossypol enhanced caspase-3 and caspase-8 expression and decreased the expression of PCNA, Bcl-2 and CD31 in tumour tissues. It suggested that cell apoptosis and inhibition of angiogenesis might contribute to the anticancer action of （-）-gossypol.
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