Immunoassay for Cadmium Detection and Quantification

作     者：GONG-LIANG LIU JU-FANG WANG ZHI-YONG LI SHI-ZHONG LIANG XIAO-NING WANG 

作者机构：School of Bioseiences & Bioengineering South China University of Technology Guangzhou 510006 Guangdong China Guangdong Inspection and Quarantine Technical Center Guangzhou 510623 Guangdong China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2009年第22卷第3期

页      面：188-193页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 120402[管理学-社会医学与卫生事业管理(可授管理学、医学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：supported by the Research Foundation of Science and Technology Project in Guangdong Province of China (No.2003C20409) Science and Technology Project in General Administration of Quality Supervision,Inspection and Quarantine of China(No. 2004IK062) 

主　　题：Cadmium ITCBE Monoclonal antibody ELISA Food Environmental samples 

摘      要：Objective To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy （GFAAS） and inductively coupled plasma atomic emission spectroscopy （ICPAES）. Methods An indirect competitive enzyme-linked immunosorbent assay （IC-ELISA） was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated. Results IC-ELISA showed an IC50 of 45.6 μg/L with a detection limit of 1.95 μg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998. Conclusion IC-ELISA can detect and quantify cadmium residue in environmental or food samples.