Role of integrin oV in sensitivity to radiotherapy of human NPC
Role of integrin oV in sensitivity to radiotherapy of human NPC作者机构：Department of Oncology Southwest Hospital Third Military Medical University Chongqing 400038 China
出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)
年 卷 期：2009年第8卷第2期
页 面：86-89页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：human NPC cell line CNE-2Z multicellular spheroids integrin αV radiosensitivity apoptosis
摘 要：Objective： To investigate whether the cell adhesion molecule integrin αV could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells via effecting intercellular effect. Methods： Human NPC cell line CNE-2Z was cultivated as three dimensional model using liquid overlay technique. Cell counting was employed to detect the radiosensitivity of CNE-2Z cells by blood cell counter before and after blocking integrin aV function; cell apoptosis was detected by flow cytometry using Annexin V/PI label. Results： Three dimensional culture model of human NPC cell line CNE-2Z cells were successfully established. The integrin αV expression of CNE-2Z multicellular spheroids elevated as the dosage of radiotherapy increased. Blocking of integrin aV function leaded to higher radiosensitivity and more apoptotic cells of CNE-2Z multicellular spheroids. Conclusion： Cell adhesion molecules integrin aV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the inhibition of cell apoptosis might be its mechanism, proved by three dimensional culture model to mimic solid tumor in vivo.
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