Regulation of HIF-1 α to Expression of N-myc Downstream Regulated Gene 1 in Colorectal Carcinoma

作     者：ZHAO Duanyi LIU Zhisu JIANG Congqing BANGOURA Gassimou WU Kailang WU Jianauo 

作者机构：Department of Colorectal Surgery Zhongnan Hospital of Wuhan University Wuhan 430071 Hubei China College of Life Sciences Wuhan University/National Key Laboratory of Virology Wuhan 430072 Hubei China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2007年第12卷第3期

页      面：563-568页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the Fund for Key Technologies R and D Pro-gramme of Hubei Province(2006AA301A03 ) 

主　　题：hypoxia inducible factor-1 α (HIF-1 α ) N-myc downstream regulated gene 1 small interfering RNA colorectal carcinoma 

摘      要：Plasmid expressing small interfering RNA （siRNA） against HIF-1α （pSilence-2.1-U6-siRNA） was constructed and transfected into LS174T cells in hypoxia *** expression of siRNA against HIF-1 α in LS174T cells, expressions of HIF-1 α and N-myc downstream regulated gene 1 （NDRG1） gene were inhibited significantly. HIF-1 cta transcripts were positive in 67.7% （42/62） and 44.4% （8/18） of colorectal adenocarcinoma and adenoma, re- spectively. The mean percentage of cells with positive hybridization of HIF-1 α mRNA increases with the development from Duke stage A to stage C＋D （p〈 0.05）. The positive staining rate of NDRG1 protein was significant higher in than that in colorectal adenoma colorectal adenocarcinoma group group （p〈 0.05）. The level of HIF-1 a transcripts was positively correlated with the level of NDRG1 protein （p 〈 0.05） during colorectal tumor progression. HIF-1α and its down stream gene NDRG1 may play roles in tumor progression of human colorectal carcinoma.