Establishment of submandibular gland allotransplantation model in miniature swine

作     者：GE Xi-yuan YU Guang-yan CAI Zhi-gang MAO Chi 

作者机构：Department of Oral and Maxillofacial Surgery Peking UniversitySchool of Stomatology Beijing 100081 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2006年第119卷第6期

页      面：482-487页

核心收录：

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：This study was supported by grants from the National Nature Science Foundation of China (No. 30340066)the Ministry of Science and Technology (No. 2003ccc00800) 

主　　题：submandibular gland transplantation miniature swine animal model 

摘      要：Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substitute. However, severe xerophthalmia can be accompanied by salivary gland dysfunction, making such autotransplantation unsuitable. Therefore, SMG allotransplantation might be a solution. The aim of this study was to assess the technical feasibility of submandibular gland allotransplantation. Methods Twelve miniature swine were randomized to serve as donors or recipients. One SMG was transplanted between a donor and a recipient. The donor SMG was revascularized by microvascular anastomosis of its vascular pedicle to the recipient lingual artery and external jugular vein. The secretory duct was implanted into the vestibule of the mouth through a subcutaneous tunnel. No ilnmunosuppressive agent was administered. The results were assessed by visual inspection of the secretion, and histopathological examination of the transplanted SMG. Results Technically, all surgical procedures were successful. Clear secretion flowed out of the duct as soon as blood supply of the transplanted submandibular gland was reestablished. The secretion of the gland lasted for 5 days. As expected, an acute rejection reaction occurred after surgery because no immunosuppressive agents were used. Secretion from the transplanted SMG ceased within 5 days. Conclusions A model of SMG allotransplantation can be established in miniature swine. The technique of submandibular gland allotransplantation is feasible.