Evaluation of Various Factors Affecting Bioconversion of L-Tyrosine to L-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology

作     者：Swati T.Gurme Shripad N.Surwase Sushama A.Patil Jyoti P.Jadhav 

作者机构：Department of BiotechnologyShivaji UniversityVidyanagarKolhapur 416004India Department of MicrobiologyShivaji UniversityKolhapur 416004India 

出 版 物：《Natural Products and Bioprospecting》 (应用天然产物（英文）)

年 卷 期：2014年第4卷第3期

页      面：141-147页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 10[医学] 

基　　金：DBT-IPLS DST-PURSE DST-SAP Karen Dulski Communications Specialist Stat-Ease Inc Stat-Ease Inc Lady Tata Memorial Trust Department of Science and Technology, Government of Kerala 

主　　题：L-DOPA L-tyrosine RSM Yarrowia lipolytica 

摘      要：3,4-Dihydroxy L-phenylalanine(L-DOPA)is considered a potent drug for the treatment of Parkinson *** and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of *** of critical components were completed by using a Plackett–Burman design,while further optimization was carried out using the Box–Behnken *** optimized factor levels predicted by the model were pH 6.1,1.659 g L^(-1)yeast extract,1.491 g L^(-1)L-tyrosine and 0.0290 g L^(-1)*** predicted yield of L-DOPA with these levels was 1.319 g L^(-1),while actual yield obtained was 1.273 g L^(-1).The statistical analysis revealed that model is significant with F value 19.55 and R2 value *** process resulted in a 3.594-fold increase in the yield of LDOPA.L-DOPA was confirmed by HPTLC and HPLC ***,Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.