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The new approach of standardization of capillary electrophoresis

The new approach of standardization of capillary electrophoresis

作     者:JOSEF Havel 

作者机构:Department of Analytical Chemistry 

出 版 物:《Science China Chemistry》 (中国科学(化学英文版))

年 卷 期:2005年第48卷第Z1期

页      面:14-19页

核心收录:

学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基  金:supported by the National Natural Science Foundation of China(Grant No.20445002) 

主  题:capillary electrophoresis, standardization, precision, qualitative and quantitative analysis, wavelet transform. 

摘      要:In this paper, we develope the new standardization methods to eliminate the influence in capillary electrophoresis (CE). The markers were used to determine the basis position and then correct the data of sample by the migration time of standard sample, and make the migration time of samples consistent with the standard sample by the criterion of the marker. The problem of time transition was corrected in this way. Then according to the peak height or peak area of the marker in the sample (peak height was used here) compared with the standard sample, the sample data was zoomed appropriately. The absorbance error was made to be *** wavelet de-noise method was also used to make the data smooth and get a good baseline.

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